Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [WM59] to CD31 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-CD31 antibody [WM59] - BSA and Azide free
    See all CD31 primary antibodies

  • Description

    Mouse monoclonal [WM59] to CD31 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: HUVEC cells. Flow Cyt: HUVEC cells.

  • General notes

    ab252265 is the carrier-free version of ab218. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252265 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Images

  • Immunocytochemistry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
    Immunocytochemistry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC cells labelling CD31 with ab218 at 1/100 dilution (10.62µg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control cell: HEK-293 (PMID: 27097314).

    Negative control 1: ab218 at a 1/100 dilution (10.62µg/ml) followed by ab150080 at a 1/500 dilution (4µg/ml).

    Negative control 2: ab179513 at a 1/500 dilution (4µg/ml) followed by ab150113 at a 1/1000 dilution (4µg/ml).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218).

  • Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
    Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)

    Flow cytometric analysis of HEK 293 (human embryonic kidney epithelial cell) (Left panel) / HUVEC (human umbilical vein endothelial cell) (Right panel) cells labelling CD31 with ab218 at 1/1000 dilution (1.062µg/ml) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK 293 (PMID: 27097314).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218).

  • Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
    Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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