Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Oct-2 antibody [9A2] (ab243153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [9A2] to Oct-2
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Oct-2 antibody [9A2]
    See all Oct-2 primary antibodies

  • Description

    Rat monoclonal [9A2] to Oct-2

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos, Raji, Daudi and A20 whole cell lysate. Mouse spleen and human tonsil tissue lysate. IHC-P: Human tonsil and spleen tissue. Mouse and rat spleen tissue. Flow Cyt: Raji cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    9A2

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Oct-2 antibody [9A2] (ab243153)
    Western blot - Anti-Oct-2 antibody [9A2] (ab243153)
    All lanes : Anti-Oct-2 antibody [9A2] (ab243153) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 3 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
    Lane 4 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
    Lane 5 : Human tonsil tissue lysate
    Lane 6 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 51 kDa



    The molecular weight observed is consistent with what has been described in the literature (PMID: 3265124).

    Exposure times: Lanes 1-3: 3 seconds; Lane 4: 3 minutes; Lane 5: 15 seconds; Lane 6: 44 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (ab243153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (ab243153)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Oct-2 with ab243153 at 2.015µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat spleen (PMID: 11280769). The section was incubated with ab243153 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (ab243153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (ab243153)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Oct-2 with ab243153 at 2/015µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse spleen (PMID: 11280769). The section was incubated with ab243153 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (ab243153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (ab243153)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Oct-2 with ab243153 at 2.015µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human spleen (PMID: 11280769). The section was incubated with ab243153 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (ab243153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (ab243153)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Oct-2 with ab243153 at 2.015µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human tonsil (PMID: 11280769). The section was incubated with ab243153 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Flow Cytometry - Anti-Oct-2 antibody [9A2] (ab243153)
    Flow Cytometry - Anti-Oct-2 antibody [9A2] (ab243153)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling Oct-2 with ab243153 at 1.343µg/ml (red) compared with Rat monclonal IgG (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Oct-2 antibody [9A2] (ab243153)
    Anti-Oct-2 antibody [9A2] (ab243153)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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