Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12482] to Oct-2 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Oct-2 antibody [EPR12482] - BSA and Azide free
    See all Oct-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12482] to Oct-2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The Oct-2 targeted by ab179808 is POU domain, class 2, transcription factor 2 (SwissProt ID: P09086).

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Oct-2. The exact sequence is proprietary.
    Database link: P09086

  • Positive control

    • WB: Raji whole cell lysate. Rat and mouse brain tissue lysates; IP: Raji whole cell lysate; ICC/IF: Ramos; Flow-cyt: Raji cells.

  • General notes

    Ab250114 is the carrier-free version of ab179808. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250114 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)
    Western blot - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)
    All lanes : Anti-Oct-2 antibody [EPR12482] (ab179808) at 1/5000 dilution (Purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    The observed bands are consistent with what are described in PMID: 26993806.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179808).

  • Immunoprecipitation - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)
    Immunoprecipitation - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)

    Purified ab179808 at 1/30 dilution (2ug) immunoprecipitating Oct-2 in Raji whole cell lysate.
    Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (10µg)
    Lane 2 (+): ab179808 + Raji whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab179808 in Raji whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 58 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179808).

  • Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)
    Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)
    Immunocytochemistry/ Immunofluorescence analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling Oct-2 with Purified ab179808 at 1/200 (2.7 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179808).
  • Flow Cytometry - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)
    Flow Cytometry - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)
    Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling Oct-2 with Purified ab179808 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179808).
  • Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)
    Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (ab250114)

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