Human Oct4 ELISA Kit (ab235653)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 596 pg/ml
- Range: 1.76 ng/ml - 112.5 ng/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Oct4 ELISA Kit
See all Oct4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 8 9.8% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
596 pg/ml -
Range
1.76 ng/ml - 112.5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 102 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Oct4 ELISA Kit (ab235653) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Oct4 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Oct4 with 596 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Oct4 Capture Antibody 1 x 600µl 10X Human Oct4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human Oct4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Tissue specificity
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. -
Sequence similarities
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Developmental stage
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. -
Domain
The POU-specific domain mediates interaction with PKM2. -
Post-translational
modificationsSumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. -
Cellular localization
Nucleus. Expressed in a diffuse and slightly punctuate pattern. - Information by UniProt
-
Alternative names
- Octamer binding transcription factor 4
- MGC22487
- Oct 3
see all -
Database links
- Entrez Gene: 5460 Human
- Omim: 164177 Human
- SwissProt: Q01860 Human
- Unigene: 249184 Human
- Unigene: 632482 Human
- Unigene: 646545 Human
Images
-
Other - Human Oct4 ELISA Kit (ab235653)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
. Example of human Oct4 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Oct4 in NCCIT cell extract based on a 50 µg/mL extract loadThe concentrations of Oct4 were measured in duplicate and interpolated from the Oct4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Oct4 concentration was determined to be 26.7 ng/mL in NCCIT cell extract.
-
Sandwich ELISA - Human Oct4 ELISA Kit (ab235653)To learn more about the advantages of recombinant antibodies see here.