Ab51363 at 1:10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1:200 dilution.

Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution + HEK293 whole cell lysate at 50 µg

Secondary
Anti mouse IgG antibody at 1/2500 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.