Anti-Oct4 antibody (ab134218)
Key features and details
- Chicken polyclonal to Oct4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgY
Overview
-
Product name
Anti-Oct4 antibody
See all Oct4 primary antibodies -
Description
Chicken polyclonal to Oct4 -
Host species
Chicken -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Pig, Chimpanzee, Chinese hamster
-
Immunogen
Synthetic peptide within Human Oct4 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
-
Positive control
- This antibody gave a positive signal in the following whole cell lysates: E14Tg2a; F9. IF/ICC: mES cell line.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 3% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
Purity
Protein L purified -
Purification notes
Affinity Purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab134218)
ICC/IF image of ab134218 stained mES cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab134218, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1:250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
-
Western blot - Anti-Oct4 antibody (ab134218)All lanes : Anti-Oct4 antibody (ab134218) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
