Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Oct-2 antibody [EPR12482] (ab179808)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12482] to Oct-2
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Oct-2 antibody [EPR12482]
    See all Oct-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12482] to Oct-2

  • Host species

    Rabbit

  • Specificity

    The Oct-2 targeted by ab179808 is POU domain, class 2, transcription factor 2 (SwissProt ID: P09086).

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Oct-2. The exact sequence is proprietary.
    Database link: P09086

  • Positive control

    • WB: Raji whole cell lysate. Rat and mouse brain tissue lysates; IP: Raji whole cell lysate; ICC/IF: Ramos; Flow-cyt: Raji cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12482

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Oct-2 antibody [EPR12482] (ab179808)
    Western blot - Anti-Oct-2 antibody [EPR12482] (ab179808)
    All lanes : Anti-Oct-2 antibody [EPR12482] (ab179808) at 1/5000 dilution (Purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

    The observed bands are consistent with what are described in PMID: 26993806.

  • Immunoprecipitation - Anti-Oct-2 antibody [EPR12482] (ab179808)
    Immunoprecipitation - Anti-Oct-2 antibody [EPR12482] (ab179808)

    Purified ab179808 at 1/30 dilution (2ug) immunoprecipitating Oct-2 in Raji whole cell lysate.
    Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (10µg)
    Lane 2 (+): ab179808 + Raji whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab179808 in Raji whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 58 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody [EPR12482] (ab179808)
    Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody [EPR12482] (ab179808)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling Oct-2 with purified ab179808 at 1/200 (2.7 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Oct-2 antibody [EPR12482] (ab179808)
    Flow Cytometry - Anti-Oct-2 antibody [EPR12482] (ab179808)

    Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling Oct-2 with purified ab179808 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-Oct-2 antibody [EPR12482] (ab179808)
    Anti-Oct-2 antibody [EPR12482] (ab179808)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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