HRP Anti-Oct4 antibody (ab196586)
Key features and details
- HRP Rabbit polyclonal to Oct4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Oct4 antibody
See all Oct4 primary antibodies -
Description
HRP Rabbit polyclonal to Oct4 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab20650) -
Positive control
- WB: Hues7 whole cell lysate. IHC-P: Human testis (seminoma) tissue.
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Affinity Purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Oct4 antibody (ab196586)HRP Anti-Oct4 antibody (ab196586) at 1/5000 dilution + HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196586 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Oct4 antibody (ab196586)IHC image of 38261 staining in a section of formalin-fixed paraffin-embedded human testis (seminoma)*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196586 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
