Anti-CD133 antibody [mAbcam27699] (ab27699)
![Anti-CD133 antibody [mAbcam27699] (ab27699)](https://www.abcam.com/ps/products/27/ab27699/Images/ab27699-449553-anti-cd133-antibody-western-blot-kidney-mouse.jpg "Anti-CD133 antibody [mAbcam27699] (ab27699)")
Key features and details
- Mouse monoclonal [mAbcam27699] to CD133
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2b
Overview
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Product name
Anti-CD133 antibody [mAbcam27699]
See all CD133 primary antibodies -
Description
Mouse monoclonal [mAbcam27699] to CD133 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse CD133.
Read Abcam's proprietary immunogen policy (Peptide available as ab31714.) -
Positive control
- WB: Mouse kidney tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam27699 -
Myeloma
Sp2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-CD133 antibody [mAbcam27699] (ab27699)Anti-CD133 antibody [mAbcam27699] (ab27699) at 5 µg + Mouse kidney tissue lysate at 20 µg
Secondary
HRP conjugated goat anti-mouse at 1/5000 dilution
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible IgG)This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab27699 overnight at 4°C. Antibody binding was detected using a goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
