Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP275] to SOX10 - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SOX10 antibody [SP275] - BSA and Azide free
    See all SOX10 primary antibodies

  • Description

    Rabbit monoclonal [SP275] to SOX10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human melanoma, and breast tissue; WB: A-375 cell lysate; Flow Cyt: A-375 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab239751 is the carrier-free version of ab227684. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239751 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling SOX10 with ab227684 at 1/100 dilution (4.09 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227684)
  • Flow Cytometry - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
    Flow Cytometry - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)

    Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227684 at 1:400 dilution (1.02 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227684).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)

    Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227684 at 1:50 (8.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227684).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for SOX10 using ab227684 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227684).

  • Flow Cytometry - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
    Flow Cytometry - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)

    Flow Cytometry analysis of A-375 (human malignant melanoma cell line) cells labeling SOX10 with ab227684 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227684).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)

    Formalin-fixed, paraffin-embedded human breast tissue stained for SOX10 using ab227684 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227684).

  • Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
    Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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