Overlay histogram showing MCF-7 cells stained with ab7168 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7168, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Overlay histogram showing HUVEC cells stained with ab7168 (blue line). Red line was isotype control mouse IgG₁. Black line was an irrelevant mouse IgG₁ used under the same conditions. Controls and anti-human ß1-integrin at 0.4 µg/100000 cells (1 hr 4°C).