Anti-STELLAR antibody (ab136278)
Key features and details
- Chicken polyclonal to STELLAR
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgY
Overview
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Product name
Anti-STELLAR antibody
See all STELLAR primary antibodies -
Description
Chicken polyclonal to STELLAR -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Mouse
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Immunogen
Synthetic peptide within Human STELLAR aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
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Positive control
- In western blot, ab136278 gave a positive signal in the following whole cell lysates: mouse embryonic germ cells; E14Tg2a. This antibody gave a positive result when used in the following methanol fixed cell lines: Mouse embryonic stem cells.
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General notes
This product was previously labelled as Stella
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-STELLAR antibody (ab136278)All lanes : Anti-STELLAR antibody (ab136278) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate (ab59325)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab136278)ab136278 stained Mouse Embryonic Stem cells cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab136278 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
