Anti-STELLAR antibody (ab19878)
Key features and details
- Rabbit polyclonal to STELLAR
- Suitable for: IHC-Fr, WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-STELLAR antibody
See all STELLAR primary antibodies -
Description
Rabbit polyclonal to STELLAR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Mouse STELLAR aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23324) -
General notes
This product was previously labelled as Stella
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab19878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-Fr Use a concentration of 4 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
May play a role in maintaining cell pluripotentiality. -
Tissue specificity
Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors. -
Developmental stage
Expressed in fetal ovary. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 73708 Mouse
- Entrez Gene: 297576 Rat
- SwissProt: Q8QZY3 Mouse
- SwissProt: Q6IMK0 Rat
- Unigene: 27982 Mouse
- Unigene: 79056 Rat
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Form
Stella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage. -
Alternative names
- Compaction associated protein 1 antibody
- Developmental pluripotency associated 3 antibody
- Developmental pluripotency associated protein 3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Frozen sections) - Anti-STELLAR antibody (ab19878)This image is courtesy of Petra Hajkova, University of CambridgeThe image shows ab19878 staining of a crysosection of mouse embryonic genital ridges (E14.5). The samples were fixed overnight in 4% paraformaldehyde, permeabilised with 0.1% Triton and stained overnight at 4 degrees. 4µg/ml of antibody was used. Staining was in the nucleus and cytoplasm of Oct4-positive cells. The blue fluorescence is DAPI staining of DNA.
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Western blot - Anti-STELLAR antibody (ab19878)All lanes : Anti-STELLAR antibody (ab19878) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab37113 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.
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Western blot - Anti-STELLAR antibody (ab19878)All lanes : Anti-STELLAR antibody (ab19878) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 4 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate with Mouse STELLAR peptide (ab23324) at 2 µg/ml
Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse STELLAR peptide (ab23324) at 2 µg/ml
Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Mouse STELLAR peptide (ab23324) at 2 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)This image is courtesy of Rodríguez A et al., PLoS One. 2012;7(11):e49079. doi: 10.1371/journal.pone.0049079. Epub 2012 Nov 8.; Fig 4.; November 8, 2012, PLoS ONE 7(11): e49079. doi:10.1371/journal.pone.0049079Immunofluorescence analysis of porcine induced pluripotent stem cells cultured in 2i + LIF medium, staining STELLAR with ab19878.
Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked in 5% BSA. Cells were incubated with primary antibody (1/60) overnight at 4°C.
Protocols
References (20)
ab19878 has been referenced in 20 publications.
- Kim Y et al. Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration. Nat Commun 11:1994 (2020). PubMed: 32332736
- El-Behery EI et al. The efficacy of chronic zinc oxide nanoparticles using on testicular damage in the streptozotocin-induced diabetic rat model. Acta Histochem 121:84-93 (2019). IHC-P ; Rat . PubMed: 30413282
- Ando Y et al. Modulation of adhesion microenvironment using mesh substrates triggers self-organization and primordial germ cell-like differentiation in mouse ES cells. APL Bioeng 3:016102 (2019). PubMed: 31069335
- Leopardo NP & Vitullo AD Early embryonic development and spatiotemporal localization of mammalian primordial germ cell-associated proteins in the basal rodent Lagostomus maximus. Sci Rep 7:594 (2017). IHC-P ; Other species . PubMed: 28377629
- Xie F et al. Obesity-Dependent Increases in Oocyte mRNAs Are Associated With Increases in Proinflammatory Signaling and Gut Microbial Abundance of Lachnospiraceae in Female Mice. Endocrinology 157:1630-43 (2016). PubMed: 26881311
Images
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Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Frozen sections) - Anti-STELLAR antibody (ab19878) This image is courtesy of Petra Hajkova, University of Cambridge
The image shows ab19878 staining of a crysosection of mouse embryonic genital ridges (E14.5). The samples were fixed overnight in 4% paraformaldehyde, permeabilised with 0.1% Triton and stained overnight at 4 degrees. 4µg/ml of antibody was used. Staining was in the nucleus and cytoplasm of Oct4-positive cells. The blue fluorescence is DAPI staining of DNA.
-
Western blot - Anti-STELLAR antibody (ab19878)All lanes : Anti-STELLAR antibody (ab19878) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab37113 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.
-
Western blot - Anti-STELLAR antibody (ab19878)All lanes : Anti-STELLAR antibody (ab19878) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 4 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate with Mouse STELLAR peptide (ab23324) at 2 µg/ml
Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse STELLAR peptide (ab23324) at 2 µg/ml
Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Mouse STELLAR peptide (ab23324) at 2 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
-
Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878) This image is courtesy of Rodríguez A et al., PLoS One. 2012;7(11):e49079. doi: 10.1371/journal.pone.0049079. Epub 2012 Nov 8.; Fig 4.; November 8, 2012, PLoS ONE 7(11): e49079. doi:10.1371/journal.pone.0049079
Immunofluorescence analysis of porcine induced pluripotent stem cells cultured in 2i + LIF medium, staining STELLAR with ab19878.
Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked in 5% BSA. Cells were incubated with primary antibody (1/60) overnight at 4°C.
