Anti-CROP antibody (ab79231)
Key features and details
- Rabbit polyclonal to CROP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CROP antibody
See all CROP primary antibodies -
Description
Rabbit polyclonal to CROP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Human CROP aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87825) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; U2OS; Jurkat; HEK293; HepG2; Caco2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab79231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing. -
Tissue specificity
Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung. -
Sequence similarities
Belongs to the Luc7 family. -
Post-translational
modificationsPhosphorylated in vitro by SRPK1, SRPK2 and CLK1. -
Cellular localization
Nucleus speckle. The subnuclear localization is affected by cisplatin. - Information by UniProt
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Database links
- Entrez Gene: 539955 Cow
- Entrez Gene: 51747 Human
- Entrez Gene: 67684 Mouse
- Entrez Gene: 100172991 Orangutan
- Omim: 609434 Human
- SwissProt: Q3SX41 Cow
- SwissProt: O95232 Human
- SwissProt: Q5SUF2 Mouse
see all -
Alternative names
- cAMP regulatory element-associated protein 1 antibody
- Cisplatin resistance-associated overexpressed protein antibody
- Cisplatin resistance-associated-overexpressed protein antibody
see all
Images
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Western blot - Anti-CROP antibody (ab79231)All lanes : Anti-CROP antibody (ab79231) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
The 60 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to CROP. -
Immunocytochemistry/ Immunofluorescence - Anti-CROP antibody (ab79231)ICC/IF image of ab79231 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79231, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab79231 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-CROP antibody (ab79231)All lanes : Anti-CROP antibody (ab79231) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
The 60 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to CROP. -
Immunocytochemistry/ Immunofluorescence - Anti-CROP antibody (ab79231)ICC/IF image of ab79231 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79231, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
