Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRBC1 antibody [SP305] (ab227699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP305] to TRBC1
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TRBC1 antibody [SP305]
    See all TRBC1 primary antibodies

  • Description

    Rabbit monoclonal [SP305] to TRBC1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil, lymph node, spleen, thymus, colon, stomach, urinary bladder, and lung squamous cell carcinoma tissue WB: Ramos, Jurkat and HEK-293 cell lysate

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     This product was previously labelled as T-cell receptor beta-1 chain C region

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by Protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP305

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling TRBC1 with ab227699 at 1/100 dilution (2.28 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-TRBC1 antibody [SP305] (ab227699)
    Western blot - Anti-TRBC1 antibody [SP305] (ab227699)
    All lanes : Anti-TRBC1 antibody [SP305] (ab227699) at 0.228 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) cell lysate
    Lane 3 : HEK-293 ( Human embryonic kidney epithelial cell) cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 20 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling TRBC1 with ab227699 at 1/100 dilution (2.28 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

    Formalin-fixed, paraffin-embedded human urinary bladder tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

    Formalin-fixed, paraffin-embedded human colon tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

    Formalin-fixed, paraffin-embedded human spleen stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

    Formalin-fixed, paraffin-embedded human lymph node tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.

  • Anti-TRBC1 antibody [SP305] (ab227699)
    Anti-TRBC1 antibody [SP305] (ab227699)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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