Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸ 1 ml 465 705 ₸ 500 µl 385 296 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP305] to TRBC1
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-TRBC1 antibody [SP305]
See all TRBC1 primary antibodies
Description

Rabbit monoclonal [SP305] to TRBC1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil, lymph node, spleen, thymus, colon, stomach, urinary bladder, and lung squamous cell carcinoma tissue WB: Ramos, Jurkat and HEK-293 cell lysate
General notes

FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product was previously labelled as T-cell receptor beta-1 chain C region

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by Protein A/G.
Clonality

Monoclonal
Clone number

SP305
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling TRBC1 with ab227699 at 1/100 dilution (2.28 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-TRBC1 antibody [SP305] (ab227699)

All lanes : Anti-TRBC1 antibody [SP305] (ab227699) at 0.228 µg/ml

Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) cell lysate
Lane 3 : HEK-293 ( Human embryonic kidney epithelial cell) cell lysate

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 20 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer and concentration: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling TRBC1 with ab227699 at 1/100 dilution (2.28 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Formalin-fixed, paraffin-embedded human thymus tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Formalin-fixed, paraffin-embedded human urinary bladder tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Formalin-fixed, paraffin-embedded human stomach tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Formalin-fixed, paraffin-embedded human colon tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Formalin-fixed, paraffin-embedded human spleen stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRBC1 antibody [SP305] (ab227699)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TRBC1 using ab227699 at 1/100 dilution in immunohistochemical analysis.
Anti-TRBC1 antibody [SP305] (ab227699)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRBC1 antibody [SP305] (ab227699)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Images

Alternative products to Anti-TRBC1 antibody [SP305] (ab227699)

Anti-TRBC1 antibody (ab18862)