Antibodies, Proteins, Kits and Reagents for Life Science

Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

Key features and details

Expression system: HEK 293 cells
Purity: > 95% SDS-PAGE
Endotoxin level:
Active: Yes
Tags: Fc tag C-Terminus
Suitable for: Functional Studies, SDS-PAGE, Flow Cyt, ELISA

Alternative names
- DKFZp667A205
- ENSMUSG00000071552
- FLJ39873
- RGD1563191
- T cell immunoreceptor with Ig and ITIM domains
- TIGIT
- TIGIT_HUMAN
- V-set and immunoglobulin domain containing 9
- V-set and immunoglobulin domain-containing protein 9
- V-set and transmembrane domain containing 3
- V-set and transmembrane domain-containing protein 3
- VSIG9
- VSTM3
- Washington University cell adhesion molecule
- WUCAM
see all
Function

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Tissue specificity

Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level).
Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Domain

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Cellular localization

Cell membrane.
Target information above from: UniProt accession Q495A1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

ELISA - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

Immobilized Recombinant human Poliovirus Receptor/PVR protein (ab220561) at 5 μg/mL (100 μL/well) can bind ab223110 with a linear range of 0.5-8 ng/mL.
Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

Loaded ab223110 on Protein A Biosensor, can bind Recombinant human Poliovirus Receptor/PVR protein (ab155723) with an affinity constant of 0.92 μM as determined in BLI assay.
SDS-PAGE - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

ab223110 analyzed by reduced SDS-PAGE and stained overnight with Coomassie Blue. The protein migrates as 42-50 kDa under reducing conditions due to glycosylation.
Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

FACS assay shows that recombinant Human TIGIT Protein, Mouse IgG2a Fc Tag (ab223110) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/ml
Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT (ab223110): Biotinylated Human CD112, Fc Tag (ab246048) binding reactions. Background was subtracted from data points before curve fitting.

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