Anti-eIF2A antibody (ab235147)
Key features and details
- Rabbit polyclonal to eIF2A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF2A antibody
See all eIF2A primary antibodies -
Description
Rabbit polyclonal to eIF2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: eIF2A human protein over HEK-293 expressing lysate; Daudi and HeLa whole cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody (ab235147)
4% paraformaldehyde-fixed, 0.3%Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling elF2A using ab235147 at 5 µg/mL overnight at 4ºC in ICC/IF analysis. Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 µg/mL for 1 hour at room temperature.
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Western blot - Anti-eIF2A antibody (ab235147)All lanes : Anti-eIF2A antibody (ab235147) at 5 µg/ml
Lane 1 : Daudi whole cell lysate at 35 µg
Lane 2 : MCF7 whole cell lysate at 35 µg
Lane 3 : HeLa whole cell lysate
Secondary
All lanes : Donkey anti-Goat HRP at 1/40000 dilution
Predicted band size: 65 kDa
Exposure time: 35 seconds
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Western blot - Anti-eIF2A antibody (ab235147)All lanes : Anti-eIF2A antibody (ab235147) at 1/1000 dilution
Lane 1 : eIF2A human protein over HEK293 expressing lysate
Lane 2 : HEK293 lysate (empty vector)
Secondary
All lanes : Donkey Fab2 anti-Goat HRP at 1/70000 dilution
Predicted band size: 65 kDa
Exposure time: 15 seconds
