Recombinant human SMYD4 protein (Active) (ab271747)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human SMYD4 protein (Active) -
Biological activity
Assay Conditions: Add 50 µl reaction mix (100 M S-adenosylmethionine, SMYD4 enzyme, and HMT buffer 7Z’) to wells coated with the substrate. Incubate 3-16 hours. Add antibody against methylated K4 residue of histone H3, incubate 1 hour. Then, add secondary HRP-labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminescence. -
Purity
>= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DLPVDEWKSYLLQKWASLPTSVQVTISTAETLRDIFLHSSSLLQPEDELF LKRLSKGYLVGKDSDAPLFYREEGNKKFQEKDYTGAAVLYSKGVSHSRPN TEDMSLCHANRSAALFHLGQYETCLKDINRAQTHGYPERLQPKIMLRKAE CLVALGRLQEASQTISDLERNFTATPALADVLPQTLQRNLHRLKMKMQEK DSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVDPLKGRCLVATK DILPGELLVQEDAFVSVLNPGELPPPHHGLDSKWDTRVTNGDLYCHRCLK HTLATVPCDGCSYAKYCSQECLQQAWELYHRTECPLGGLLLTLGVFCHIA LRLTLLVGFEDVRKIITKLCDKISNKDICLPESNNQVKTLNYGLGESEKN GNIVETPIPGCDINGKYENNYNAVFNLLPHTENHSPEHKFLCALCVSALC RQLEAASLQAIPTERIVNSSQLKAAVTPELCPDVTIWGVAMLRHMLQLQC NAQAMTTIQHTGPKGSIVTDSRQVRLATGIFPVISLLNHSCSPNTSVSFI STVATIRASQRIRKGQEILHCYGPHKSRMGVAERQQKLRSQYFFDCACPA CQTEAHRMAAGPRWEAFCCNSCGAPMQGDDVLRCGSRSCAESAVSRDHLV SRLQDLQQQVRVAQKLLRDGELERAVQRLSGCQRDAESFLWAEHAVVGEI ADGLARACAALGDWQKSATHLQRSLYVVEVRHGPSSVEMGHELFKLAQIF FNGFAVPEALSTIQKAEEVLSLHCGPWDDEIQELQKMKSCLLDLPPTPVG PAL -
Predicted molecular weight
90 kDa including tags -
Amino acids
2 to 804 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
- KIAA1936
- SET and MYND domain containing 4
- SET and MYND domain-containing protein 4
Images
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Functional Studies - Recombinant human SMYD4 protein (Active) (ab271747)Specific activity of ab271747.
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SDS-PAGE - Recombinant human SMYD4 protein (Active) (ab271747)SDS-PAGE analysis of 3 µg ab271747.