Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PI3 Kinase p110 beta antibody [Y384] - BSA and Azide free (ab247274)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y384] to PI3 Kinase p110 beta - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PI3 Kinase p110 beta antibody [Y384] - BSA and Azide free
    See all PI3 Kinase p110 beta primary antibodies

  • Description

    Rabbit monoclonal [Y384] to PI3 Kinase p110 beta - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises PI3-Kinases (PI3-Ks). 

  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details
    Unsuitable for: ICC or IHC

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Cow, Cat, Dog, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247274 is the carrier-free version of ab32569 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247274 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] - BSA and Azide free (ab247274)
    Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] - BSA and Azide free (ab247274)

    This data was developed using ab32569, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (40 µg)

    Lane 2: PI3 Kinase p110 beta knockout HAP1 cell lysate (40 µg)

    Lane 3: Jurkat cell lysate (40 µg)

    Lane 4: K562 cell lysate (40 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32569 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32569 was shown to recognize PI3 Kinase p110 beta when PI3 Kinase p110 beta knockout samples were used, along with additional cross-reactive bands. Wild-type and PI3 Kinase p110 beta knockout samples were subjected to SDS-PAGE. Ab32569 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] - BSA and Azide free (ab247274)
    Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] - BSA and Azide free (ab247274)
    Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) at 1/500 dilution + Jurkat cell lysate

    Predicted band size: 123 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32569, the same antibody clone in a different buffer formulation.

     

  • Anti-PI3 Kinase p110 beta antibody [Y384] - BSA and Azide free (ab247274)
    Anti-PI3 Kinase p110 beta antibody [Y384] - BSA and Azide free (ab247274)

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