Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRP6 antibody [EPR22910-39] (ab231779)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22910-39] to LRP6
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-LRP6 antibody [EPR22910-39]
    See all LRP6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22910-39] to LRP6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1 (WT), HeLa, HepG2 and HEK-293T whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22910-39

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LRP6 antibody [EPR22910-39] (ab231779)
    Western blot - Anti-LRP6 antibody [EPR22910-39] (ab231779)
    All lanes : Anti-LRP6 antibody [EPR22910-39] (ab231779) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : LRP6 knockout HAP1 whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 5 : 293T (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 180 kDa
    Observed band size: 180-210 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 16989816, 16263759, 28341812).  ab231779 was shown to specifically react with LRP6 in wild-type HAP1 cells as signal was lost in LRP6 knockout cells. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab231779 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ instrument using the ECL technique.  This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 3 mins; Lanes 3: 26 secs; Lanes 4-5: 3 mins.

  • Western blot - Anti-LRP6 antibody [EPR22910-39] (ab231779)
    Western blot - Anti-LRP6 antibody [EPR22910-39] (ab231779)
    All lanes : Anti-LRP6 antibody [EPR22910-39] (ab231779) at 1/1000 dilution

    Lane 1 : His-tagged human LRP5 recombinant protein, 10 ng
    Lane 2 : His-tagged human LRP6 recombinant protein, 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 180 kDa
    Observed band size: 18,36 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    The 36 KD band observed is consistent with the dimer of human LRP6 recombinant protein (aa 1476-1600).

  • Anti-LRP6 antibody [EPR22910-39] (ab231779)
    Anti-LRP6 antibody [EPR22910-39] (ab231779)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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