Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸ 10 µl 137 366 ₸

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Anti-LRP6 antibody [EPR2423(2)] (ab134146)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2423(2)] to LRP6
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-LRP6 antibody [EPR2423(2)]
See all LRP6 primary antibodies
Description

Rabbit monoclonal [EPR2423(2)] to LRP6
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human LRP6 aa 1500-1600. The exact sequence is proprietary.
Positive control
- WB: HAP1, HeLa, HepG2, 293T, and Jurkat whole cell lysate (ab7899).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2423(2)
Isotype

IgG
Research areas

Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LRP6 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab134146 observed at 220 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab134146 was shown to recognize LRP6 when LRP6 knockout samples were used, along with additional cross-reactive bands. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab134146 and ab18058 (loading control to Vinculin) were diluted 1/10000 and 1/1000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146)

All lanes : Anti-LRP6 antibody [EPR2423(2)] (ab134146) at 1/1000 dilution

Lane 1 : HeLa cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : 293T cell lysates
Lane 4 : Jurkat cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 180 kDa
Anti-LRP6 antibody [EPR2423(2)] (ab134146)

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