Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (ab232484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2423(2)] to LRP6 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free
    See all LRP6 primary antibodies

  • Description

    Rabbit monoclonal [EPR2423(2)] to LRP6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HeLa, HepG2, 293T, and Jurkat whole cell lysate (ab7899).

  • General notes

    ab232484 is the carrier-free version of ab134146. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232484 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (ab232484)
    Western blot - Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (ab232484)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: LRP6 knockout HAP1 cell lysate (20 µg) 
    Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (20 µg)
    Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab134146 observed at 220 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab134146 was shown to recognize LRP6 when LRP6 knockout samples were used, along with additional cross-reactive bands. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab134146 and ab18058 (loading control to Vinculin) were diluted 1/10000 and 1/1000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134146).

  • Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (ab232484)
    Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (ab232484)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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