Anti-LRP6 antibody [EPR22910-39] - BSA and Azide free (ab256476)
![Anti-LRP6 antibody [EPR22910-39] - BSA and Azide free (ab256476)](https://www.abcam.com/ps/products/256/ab256476/Images/ab256476-352872-ab231779352731WB1antilrp6antibody-pbs-onlyepr2291039westernblothuman-ko.jpg "Anti-LRP6 antibody [EPR22910-39] - BSA and Azide free (ab256476)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22910-39] to LRP6 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-LRP6 antibody [EPR22910-39] - BSA and Azide free
See all LRP6 primary antibodies -
Description
Rabbit monoclonal [EPR22910-39] to LRP6 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1 (WT), HeLa, HepG2 and HEK-293T whole cell lysates.
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General notes
Ab256476 is the carrier-free version of ab231779. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256476 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22910-39 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LRP6 antibody [EPR22910-39] - BSA and Azide free (ab256476)All lanes : Anti-LRP6 antibody [EPR22910-39] (ab231779) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LRP6 knockout HAP1 whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 5 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 180 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231779).
The molecular weight observed is consistent with what has been described in the literature (PMID: 16989816, 16263759, 28341812). ab231779 was shown to specifically react with LRP6 in wild-type HAP1 cells as signal was lost in LRP6 knockout cells. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab231779 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ instrument using the ECL technique. This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 3 mins; Lanes 3: 26 secs; Lanes 4-5: 3 mins.
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![Anti-LRP6 antibody [EPR22910-39] - BSA and Azide free (ab256476)](https://www.abcam.com/ps/products/256/ab256476/Images/ab256476-2-benefits-of-recombinant-antibodies.png)
Anti-LRP6 antibody [EPR22910-39] - BSA and Azide free (ab256476)
