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Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2360Y] to LRP6 (phospho S1490)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-LRP6 (phospho S1490) antibody [EP2360Y]
    See all LRP6 primary antibodies
  • Description

    Rabbit monoclonal [EP2360Y] to LRP6 (phospho S1490)
  • Host species

    Rabbit
  • Specificity

    This antibody detects LRP6 phosphorylated on serine 1490.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 1490 of human LRP6.

  • Positive control

    • HeLa whole cell lysate (ab150035)
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP2360Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Transmembrane
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Surface Molecules

Images

  • Western blot - Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)
    Western blot - Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)
    All lanes : Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417) at 1/2000 dilution

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. The membrane was then treated with Alkaline Phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 179 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)
    Western blot - Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)
    All lanes : Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417) at 1/1000 dilution

    Lane 1 : HeLa cell
    lysates, untreated
    Lane 2 : HeLa cell
    lysates treated with Calyculin

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 179 kDa
    Observed band size: 210 kDa why is the actual band size different from the predicted?

  • Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)
    Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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