Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2360Y] to LRP6 (phospho S1490)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-LRP6 (phospho S1490) antibody [EP2360Y]
See all LRP6 primary antibodies -
Description
Rabbit monoclonal [EP2360Y] to LRP6 (phospho S1490) -
Host species
Rabbit -
Specificity
This antibody detects LRP6 phosphorylated on serine 1490. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
A phospho specific peptide corresponding to residues surrounding serine 1490 of human LRP6.
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Positive control
- HeLa whole cell lysate (ab150035)
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2360Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417) at 1/2000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. The membrane was then treated with Alkaline Phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 179 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417) at 1/1000 dilution
Lane 1 : HeLa cell
lysates, untreated
Lane 2 : HeLa cell
lysates treated with Calyculin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 179 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
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