Recombinant human GM-CSF protein (Active) (ab259376)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Cell Culture, SDS-PAGE, HPLC, MS, Functional Studies
Preparation and Storage
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Alternative names
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
see all -
Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. -
Sequence similarities
Belongs to the GM-CSF family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human GM-CSF protein (Active) (ab259376)
Fully biologicaly active as determined by dose-depended proliferation of TF-1 Cells.
ED50 is ≤ 0.1ng/mL, corresponding to a specific activity of 1.00 x 107 units/mg.
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SDS-PAGE - Recombinant human GM-CSF protein (Active) (ab259376)SDS-PAGE analysis of ab259376.
The protein is heavily glycosylated and therefore runs as a smear.
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HPLC - Recombinant human GM-CSF protein (Active) (ab259376)Purity 95% (sample was deglycosylated prior HPLC analysis; signal at RT 6.7 min corresponds to deglycosylation mix).
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Mass Spectrometry - Recombinant human GM-CSF protein (Active) (ab259376)M+/- 0 Da, pass (additional mass signals from glycan modifiactions: +203 Da HexNAc, +162 Da Hex)). Calc MW 14523.00 Da.