Anti-PIH1D1 antibody (ab238862)
Key features and details
- Rabbit polyclonal to PIH1D1
- Suitable for: IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PIH1D1 antibody -
Description
Rabbit polyclonal to PIH1D1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human PIH1D1 aa 1 to the C-terminus.
Database link: Q9NWS0-1 -
Positive control
- IHC-P: Human placenta tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIH1D1 antibody (ab238862)
Paraffin-embedded human placenta tissue stained for PIH1D1 using ab238862 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PIH1D1 antibody (ab238862)HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for PIH1D1 (green) using ab238862 at 1/200 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counterstain is DAPI.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
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Immunoprecipitation - Anti-PIH1D1 antibody (ab238862)PIH1D1 was immunoprecipitated from 0.5 mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab238862 at 1/200 dilution.
Lane 1: Control rabbit IgG IP (1 μg) in HeLa whole cell lysate.
Lane 2: ab238862 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 10 μg (Input).For Western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.