Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3899] to ARFRP1/ARP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-ARFRP1/ARP antibody [EPR3899]
    See all ARFRP1/ARP primary antibodies

  • Description

    Rabbit monoclonal [EPR3899] to ARFRP1/ARP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and HEK-293T cell lysate. Fetal liver and ovary cancer lysates ICC/IF: HeLa cells

  • General notes

     

     

    Previously labelled as ARFRP1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3899

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
    Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
    All lanes : Anti-ARFRP1/ARP antibody [EPR3899] (ab108199) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Fetal liver lysate
    Lane 4 : Ovary cancer lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
    Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARFRP1/ARP with purified ab108199 at 1/500 dilution (4.8 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
    Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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