Anti-SEC16A/SEC16 antibody (ab70722)
Key features and details
- Rabbit polyclonal to SEC16A/SEC16
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SEC16A/SEC16 antibody -
Description
Rabbit polyclonal to SEC16A/SEC16 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human SEC16A/SEC16.
Database link: O15027 -
Positive control
- Whole cell lysate from HeLa and 293T cells.
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General notes
This product was previously labelled as SEC16A
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SEC16A/SEC16 antibody (ab70722)All lanes : Anti-SEC16A/SEC16 antibody (ab70722) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 234 kDa
Observed band size: >250 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunoprecipitation - Anti-SEC16A/SEC16 antibody (ab70722)Detection of Human SEC16A/SEC16 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70722 at 3 µg/mg lysate. Subsequent Western blot detection of SEC16A/SEC16 was performed using ab70722 at 0.04 µg/ml.