Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19840] to MASH1/Achaete-scute homolog 1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free
    See all MASH1/Achaete-scute homolog 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19840] to MASH1/Achaete-scute homolog 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240385 is the carrier-free version of ab211327.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse small cell lung cancer section labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution (red). 

    Nuclear counterstain: DAPI (blue).

    Positive staining of MASH1/Achaete-scute homolog 1 on mouse small cell lung cancer.

    The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed Ascl1CreER/+; ROSA26mTmG/+ mouse thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Tyramide Signal Amplification (red). 

    Counterstain: DAPI (blue) and chick anti-GFP (green).

    ROSA26mTmG/+ mice were bred with Ascl1CreER/+ mice, in which the Ascl1 promoter drives the expression of Cre, to generate mice carrying the genotype of Ascl1CreER/+; ROSA26mTmG/+. Cre activation upon Tamoxifen administration resulted in eGFP expression from the ROSA26mTmG/+ allele. The thyroid tissue from Ascl1CreER/+; ROSA26mTmG/+ mouse was fixed and embedded in paraffin. Paraffin section was stained with rabbit anti-MASH1/Achaete-scute homolog 1 (ab211327) antibody (Red color) and chick anti-GFP antibody (Green color). Tyramide signal amplification was used for high-resolution imaging of low-abundance targets.

    The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)
    Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized NCI-H69 Human small cell lung cancer cells labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution (red).

    The nuclear counterstain is DAPI (blue).

    Nuclear MASH1/Achaete-scute homolog 1 staining on NCI-H69 cells.

    The images were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).

  • Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)
    Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)

    MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of mouse medullary thyroid cancer lysate  with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse medullary thyroid cancer lysate 10 μg (Input).

    Lane 2: ab211327 IP in mouse medullary thyroid cancer lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211327 in mouse medullary thyroid cancer lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).

  • Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)
    Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)

    MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of NCI-H69 (Human small cell lung cancer cell line) whole cell lysate  with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NCI-H69 whole cell lysate 10 μg (Input).

    Lane 2: ab211327 IP in NCI-H69 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211327 in NCI-H69 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).

  • Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)
    Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)

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