Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886)
Key features and details
- Rabbit polyclonal to MASH1/Achaete-scute homolog 1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MASH1/Achaete-scute homolog 1 antibody
See all MASH1/Achaete-scute homolog 1 primary antibodies -
Description
Rabbit polyclonal to MASH1/Achaete-scute homolog 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human MASH1/Achaete-scute homolog 1 (internal sequence). The exact sequence is proprietary.
Database link: P50553 -
Positive control
- WB: SK N SH whole cell lysate (ab3956), SH-SY5Y, PC-12 and Rat2 whole cell extracts; SH-SY5Y nuclear extract. IHC-P: Rat brain tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract
Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886)Paraffin-embedded rat brain tissue stained for MASH1/Achaete-scute homolog 1 using ab227886 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
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All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa15 % SDS-PAGE gel.
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All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab227886) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.