Anti-TCF12 antibody [7B3] (ab243149)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7B3] to TCF12
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TCF12 antibody [7B3]
See all TCF12 primary antibodies -
Description
Rat monoclonal [7B3] to TCF12 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SW620, HeLa, Raji, Jurkat and WEHI-231 cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7B3 -
Isotype
IgG2b -
Research areas
Images
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All lanes : Anti-TCF12 antibody [7B3] (ab243149) at 0.394 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 5 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 72 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?The expression molecular weight observed is consistent with what has been described in the literature (PMID: 16785505).
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
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