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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH

Anti-TCF12 antibody [7B3] (ab243149)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-TCF12 antibody [7B3] (ab243149)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7B3] to TCF12
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-TCF12 antibody [7B3]
    See all TCF12 primary antibodies
  • Description

    Rat monoclonal [7B3] to TCF12
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW620, HeLa, Raji, Jurkat and WEHI-231 cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    7B3
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other

Images

  • Western blot - Anti-TCF12 antibody [7B3] (ab243149)
    Western blot - Anti-TCF12 antibody [7B3] (ab243149)
    All lanes : Anti-TCF12 antibody [7B3] (ab243149) at 0.394 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 5 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 72 kDa
    Observed band size: 92 kDa
    why is the actual band size different from the predicted?



    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 16785505).

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

  • Anti-TCF12 antibody [7B3] (ab243149)
    Anti-TCF12 antibody [7B3] (ab243149)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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