Anti-TCF12 antibody (ab70746)
Key features and details
- Rabbit polyclonal to TCF12
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TCF12 antibody
See all TCF12 primary antibodies -
Description
Rabbit polyclonal to TCF12 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to a region between residue 1 and 50 of human TCF12 (NP_996919.1).
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Positive control
- Whole cell lysate from HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TCF12 antibody (ab70746)All lanes : Anti-TCF12 antibody (ab70746) at 1/10000 dilution
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 120 kDa, 190 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF12 antibody (ab70746)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TCF12 with ab70746 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-TCF12 antibody (ab70746)1mg whole cell lysate from HeLa cells was immunprecipitated with ab70746 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab707345 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds
