Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TCF12 antibody [7B3] - BSA and Azide free (ab252269)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7B3] to TCF12 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TCF12 antibody [7B3] - BSA and Azide free
    See all TCF12 primary antibodies

  • Description

    Rat monoclonal [7B3] to TCF12 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW620, HeLa, Raji, Jurkat and WEHI-231 cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252269 is the carrier-free version of ab243149. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-TCF12 antibody [7B3] - BSA and Azide free (ab252269)
    Western blot - Anti-TCF12 antibody [7B3] - BSA and Azide free (ab252269)
    All lanes : Anti-TCF12 antibody [7B3] (ab243149) at 0.394 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate, 20 ug
    Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate, 20 ug
    Lane 3 : Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate, 20 ug
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate, 20 ug
    Lane 5 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate, 20 ug

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 72 kDa



    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 16785505).

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243149).

  • Anti-TCF12 antibody [7B3] - BSA and Azide free (ab252269)
    Anti-TCF12 antibody [7B3] - BSA and Azide free (ab252269)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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