Anti-MIST-1 antibody (ab187978)
Key features and details
- Rabbit polyclonal to MIST-1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MIST-1 antibody
See all MIST-1 primary antibodies -
Description
Rabbit polyclonal to MIST-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MIST-1 aa 100 to the C-terminus.
Database link: Q7RTS1 -
Positive control
- IHC-P: Human pancreas, stomach, tonsil and skeletal muscle tissue; WB: Transfected HEK-293T, RPMI 8226 whole cell lysates; ICC: HepG2 whole cells.
-
General notes
Protein previously known as BHLHA15.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling MIST-1 in the nucleus of exocrine glandular cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-MIST-1 antibody (ab187978) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MIST-1 over expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 20 kDa
-
Immuncytochemical analysis of HepG2 (Human liver hepatocellular carcinoma cell line) whole cells labeling MIST-1 in the nucleoplasm and the Golgi apparatus with ab187978 at 2 µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MIST-1 in the nucleus of non-germinal center cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Anti-MIST-1 antibody (ab187978) at 0.4 µg/ml + RPMI 8226 (Human multiple myeloma cell line) whole cell lysate
Predicted band size: 20 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling MIST-1 in the nucleus of glandular cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MIST-1 in the striated muscle fiber (no positivity) with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

