Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)
![Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)](https://www.abcam.com/ps/products/254/ab254258/Images/ab254258-6-anti-kallikrein-7-klk7-antibody-epr22594-203-western-blot-skin.jpg "Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22594-203] to Kallikrein 7/KLK7
- Suitable for: WB, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Kallikrein 7/KLK7 antibody [EPR22594-203]
See all Kallikrein 7/KLK7 primary antibodies -
Description
Rabbit monoclonal [EPR22594-203] to Kallikrein 7/KLK7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P MouseRatHumanWB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse skin lysate; rat skin lysate. IHC-P: Human skin tissue; mouse skin tissue; rat skin tissue. IHC-Fr: Mouse skin tissue; rat skin tisssue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22594-203 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)All lanes : Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258) at 1/1000 dilution
Lane 1 : Mouse skin lysate
Lane 2 : Rat skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 15 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)](https://www.abcam.com/ps/products/254/ab254258/Images/ab254258-5-anti-kallikrein-7-klk7-antibody-epr22594-203-immunohistochemistry-skin-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)Immunohistochemical analysis of paraffin-embedded rat skin tissue labelling KLK7 with ab254258 at 1/500 dilution for 30 minutes at room temperature, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stratum corneum of rat skin (PMID 23999494). Hematoxylin used as a counterstain.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)](https://www.abcam.com/ps/products/254/ab254258/Images/ab254258-4-anti-kallikrein-7-klk7-antibody-epr22594-203-immunohistochemistry-skin-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)Immunohistochemical analysis of paraffin-embedded mouse skin tissue labelling KLK7 with ab254258 at 1/500 dilution for 30 minutes at room temperature, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stratum corneum of mouse skin (PMID 23999494). Hematoxylin used as a counterstain.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)](https://www.abcam.com/ps/products/254/ab254258/Images/ab254258-3-anti-kallikrein-7-klk7-antibody-epr22594-203-immunohistochemistry-skin-hunan.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)Immunohistochemical analysis of paraffin-embedded human skin tissue labelling KLK7 with ab254258 at 1/500 dilution for 30 minutes at room temperature, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stratum corneum of human skin (PMID 23999494). Hematoxylin used as a counterstain.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
-
![Immunohistochemistry (Frozen sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)](https://www.abcam.com/ps/products/254/ab254258/Images/ab254258-2-anti-kallikrein-7-klk7-antibody-epr22594-203-immunohistochemistry-frozen-skin-rat.jpg)
Immunohistochemistry (Frozen sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)Immunohistochemical analysis of 0.2% Triton X-100 permeabilised, 4% PFA fixed rat skin tissue labelling KLK7 with ab254258 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Specific staining on stratum corneum of rat skin (PMID: 23999494).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)](https://www.abcam.com/ps/products/254/ab254258/Images/ab254258-1-anti-kallikrein-7-klk7-antibody-epr22594-203-immunohistochemistry-frozen-skin-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)Immunohistochemical analysis of 0.2% Triton X-100 permeabilised, 4% PFA fixed mouse skin tissue labelling KLK7 with ab254258 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Specific staining on stratum corneum of mouse skin (PMID: 23999494).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
-
![Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)](https://www.abcam.com/ps/products/254/ab254258/Images/ab254258-7-benefits-of-recombinant-antibodies.png)
Anti-Kallikrein 7/KLK7 antibody [EPR22594-203] (ab254258)
