Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2367Y] to Met (c-Met) (phospho Y1349) - BSA and Azide free
  • Suitable for: IHC-P, Dot blot, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free
    See all Met (c-Met) primary antibodies

  • Description

    Rabbit monoclonal [EP2367Y] to Met (c-Met) (phospho Y1349) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Dot blot, WB, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247359 is the carrier-free version of ab68141.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    Western blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    All lanes : Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141) at 1/5000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h. Whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h and then treated with hepatocyte growth factor at 40ng/ml for 5 min. Whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h and then treated with hepatocyte growth factor at 40ng/ml for 5 min. Whole cell lysate. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 156 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab68141, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight of Met (c-Met) (phospho Y1349) and Met (c-Met) is different. ab51067 could only recognize the pro-Met which is 190kDa, but ab68141 recognizes theβ-subunits which is 145kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    This data was developed using ab68141, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68141 at a dilution of 1/50. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    Immunoprecipitation - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)

    This data was developed using ab68141, the same antibody clone in a different buffer formulation.

    ab68141 at 1/150 dilution immunoprecipitating Met (c-Met) (phospho Y1349) in HeLa (human cervix adenocarcinoma) whole cell lysate observed at 150 kDa (lanes 1 and 2).

    Lane 1 (input): HeLa cells starved for 24 hours, then treated with 40 ng/mL HGF for 5 minutes whole cell lysate, 10µg

    Lane 2 (+): ab68141 + HeLa cells starved for 24 hours, then treated with 40 ng/mL HGF for 5 minutes whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68141 in HeLa cells starved for 24 hours, then treated with 40 ng/mL HGF for 5 minutes whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    Western blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    All lanes : Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141) at 1/10000 dilution

    Lane 1 : A431 cell lysate, untreated
    Lane 2 : A431 cell lysate, treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 156 kDa
    Observed band size: ~150 kDa why is the actual band size different from the predicted?



    This data was developed using ab68141, the same antibody clone in a different buffer formulation.

  • Dot Blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    Dot Blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    This data was developed using ab68141, the same antibody clone in a different buffer formulation.Dot blot analysis of Met (c-Met) (phospho Y1349) phospho peptide (Lane 1), Met (c-Met) Non-phospho peptide (Lane 2), labelling Met (c-Met) (phospho Y1349) with ab68141 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)
    Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] - BSA and Azide free (ab247359)

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