Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ApoER2 antibody [EPR3326] (ab108208)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3326] to ApoER2
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-ApoER2 antibody [EPR3326]
See all ApoER2 primary antibodies
Description

Rabbit monoclonal [EPR3326] to ApoER2
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- SH-SY5Y, C6, Neuro-2a, Human fetal brain, Mouse brain, and Rat brain lysates,
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3326
Isotype

IgG
Research areas

Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)

All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ApoER2 knockout HAP1 whole cell lysate

Lysates/proteins at 40 µg per lane.

Predicted band size: 106 kDa

Lanes 1 - 2: Merged signal (red and green). Green - ab108208 observed at 106 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab108208 was shown to specifically react with ApoER2 in wild-type HAP1 cells as signal was lost in LRP8 knockout cells. Wild-type and ApoER2 knockout samples were subjected to SDS-PAGE. Ab108208 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)

ab108208 (purified) at 1/40 immunoprecipitating ApoER2 in SH-SY5Y (human neuroblastoma) whole cell lysate.

Lane 1 (input): SH-SY5Y whole cell lysate 10ug

Lane 2 (+): ab108208 + SH-SY5Y whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108208 in SH-SY5Y whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)

All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/10000 dilution (purified)

Lane 1 : SH-SY5Y (human neuroblastoma) whole cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : C6 (rat glioma) whole cell lysate
Lane 5 : Neuro-2a (mouse neuroblastoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 106 kDa
Observed band size: 130-170 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer 5% NFDM/TBST.

130kDa: Immature form;

170kDa: Glycosylated mature form. (PMID:24344333, 24532792, 24705369 and 25233900)
Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)

All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution (unpurified)

Lane 1 : SH-SY5Y cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Neuro-2a cell lysate
Lane 4 : Human Fetal Brain cell lysate
Lane 5 : Mouse Fetal Brain cell lysate
Lane 6 : Rat Fetal Brain cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 106 kDa
Anti-ApoER2 antibody [EPR3326] (ab108208)

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