Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ApoER2 antibody [EPR3326] (ab108208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3326] to ApoER2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ApoER2 antibody [EPR3326]
    See all ApoER2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3326] to ApoER2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SH-SY5Y, C6, Neuro-2a, Human fetal brain, Mouse brain, and Rat brain lysates,

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
    Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
    All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ApoER2 knockout HAP1 whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 106 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab108208 observed at 106 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab108208 was shown to specifically react with ApoER2 in wild-type HAP1 cells as signal was lost in LRP8 knockout cells. Wild-type and ApoER2 knockout samples were subjected to SDS-PAGE. Ab108208 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)
    Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)

    ab108208 (purified) at 1/40 immunoprecipitating ApoER2 in SH-SY5Y (human neuroblastoma) whole cell lysate.

    Lane 1 (input): SH-SY5Y whole cell lysate 10ug

    Lane 2 (+): ab108208 + SH-SY5Y whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108208 in SH-SY5Y whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
    Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
    All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/10000 dilution (purified)

    Lane 1 : SH-SY5Y (human neuroblastoma) whole cell lysate
    Lane 2 : Human fetal brain tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : C6 (rat glioma) whole cell lysate
    Lane 5 : Neuro-2a (mouse neuroblastoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 106 kDa
    Observed band size: 130-170 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST.

    130kDa: Immature form;

    170kDa: Glycosylated mature form. (PMID:24344333, 24532792, 24705369 and 25233900)

  • Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
    Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
    All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution (unpurified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : Neuro-2a cell lysate
    Lane 4 : Human Fetal Brain cell lysate
    Lane 5 : Mouse Fetal Brain cell lysate
    Lane 6 : Rat Fetal Brain cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 106 kDa

  • Anti-ApoER2 antibody [EPR3326] (ab108208)
    Anti-ApoER2 antibody [EPR3326] (ab108208)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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