Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22331-81] to CD8 beta - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB, IHC-Fr
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free
    See all CD8 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR22331-81] to CD8 beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-Frmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus and lymph node lysates. Flow Cyt: Mouse thymocytes cells. IP: Mouse thymus cells. IHC-Fr: Mouse thymus tissue.

  • General notes

    ab263956 is the carrier-free version of ab263946. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab263956 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22331-81

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)
    Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on T cells of mouse thymus (PMID: 12702501) is observed.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263946).

  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)
    Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Negative staining on mouse liver.

    Negative control: liver (PMID: 3145195).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263946).

  • Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)
    Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)

    CD8 beta was immunoprecipitated from 0.35 mg mouse thymus lysate 10µg with ab263946 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263946 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse thymus lysate 10µg
    Lane 2: ab263946 IP in mouse thymus lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263946 in mouse thymus lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263946).

  • Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)
    Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)

    Flow Cytometric analysis of mouse thymocytes using ab263946 at a 1/500 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab263946 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647. Secondary used is Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263946).

  • Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)
    Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free (ab263956)

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