Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD8 beta antibody [EPR22331-54] (ab228965)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22331-54] to CD8 beta
  • Suitable for: IP, IHC-P, WB, IHC-Fr
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD8 beta antibody [EPR22331-54]
    See all CD8 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR22331-54] to CD8 beta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen, lymph node and thymus lysates. IP: Mouse thymus lysate. IHC-P: Mouse spleen and thymus tissue. IHC-Fr: Mouse thymus tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22331-54

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-54] (ab228965)
    Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-54] (ab228965)

    CD8 beta was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab228965 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228965 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Mouse thymus lysate 10 μg (Input).
    Lane 2: ab228965 IP in mouse thymus lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab22896 in mouse thymus lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-54] (ab228965)
    Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-54] (ab228965)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse thymus tissue stained for CD8 beta (green) using ab228965 at 1/100 dilution in immunohistochemical analysis. The secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Specific membrane staining on T cells in the cortex of mouse thymus (PMID 12702501) is observed. The nuclear conter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (ab228965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (ab228965)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD8 beta with ab228965 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells in the cortex of mouse thymus (PMID: 12702501) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab228965 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (ab228965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (ab228965)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 beta with ab228965 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 18776164) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab228965 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-CD8 beta antibody [EPR22331-54] (ab228965)
    Western blot - Anti-CD8 beta antibody [EPR22331-54] (ab228965)
    All lanes : Anti-CD8 beta antibody [EPR22331-54] (ab228965) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse lymph node lysate
    Lane 3 : Mouse thymus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28-32 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    CD8 beta is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 11719190).

    Exposure times:
    Lanes 1-2: 37 seconds.
    Lane 3: 6 seconds.

  • Anti-CD8 beta antibody [EPR22331-54] (ab228965)
    Anti-CD8 beta antibody [EPR22331-54] (ab228965)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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