Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22507-48] to CD39 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD39 antibody [EPR22507-48] - BSA and Azide free
    See all CD39 primary antibodies

  • Description

    Rabbit monoclonal [EPR22507-48] to CD39 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta, heart and spleen lysate. IHC-P: Mouse liver and spleen tissue. ICC/IF: Mouse splenocytes. Flow Cyt: Mouse splenocytes. IP: Mouse placenta lysate.

  • General notes

    ab254006 is the carrier-free version of ab227840. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254006 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with ab227840 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse spleen (PMID: 18567823). The section was incubated with ab227840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227840).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)
    Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse splenocytes labeling CD39 with ab227840 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of mouse splenocyte. The nuclear stain is DAPI (blue). Counterstained with Anti-CD45R/B220 Antibody (Alexa Fluor® 647)  at a 1/200 dilution (red). 

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227840).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD39 with ab227840 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of mouse liver (PMID: 18567823). The section was incubated with ab227840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227840).

  • Flow Cytometry - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)
    Flow Cytometry - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)

    Flow cytometric analysis of mouse splenocytes labeling CD39 with ab227840 at 1/500. Cells were stained with rabbit IgG (Left panel) or ab227840 (Right panel). Then stained with anti-CD45R conjugated to Alexa Fluor® 647. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227840).
  • Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)
    Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)

    CD39 was immunoprecipitated from 0.35 mg mouse placenta lysate with ab227840 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227840 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse placenta lysate 10 µg (Input).
    Lane 2: ab227840 IP in mouse placenta lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227840 in mouse placenta lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227840).

  • Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)
    Anti-CD39 antibody [EPR22507-48] - BSA and Azide free (ab254006)

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