Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22691-219] to CD38 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD38 antibody [EPR22691-219] - BSA and Azide free
    See all CD38 primary antibodies

  • Description

    Rabbit monoclonal [EPR22691-219] to CD38 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Mouse splenocytes. Flow cyt: Mouse splenocytes.

  • General notes

    Ab255693 is the carrier-free version of ab235590. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255693 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Flow Cytometry - Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)
    Flow Cytometry - Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)

    Flow cytometric analysis of mouse splenocytes labeling CD38 with ab235590 at 1/500 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab235590 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235590).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)
    Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD38 with ab235590 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of mouse splenocytes. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS nstead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235590).

  • Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)
    Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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