Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)
![Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)](https://www.abcam.com/ps/products/255/ab255693/Images/ab255693-2-anti-cd38-antibody-epr22691-219-flowcytometry-splenocytes-cd45r-mouse.jpg "Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22691-219] to CD38 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
Overview
-
Product name
Anti-CD38 antibody [EPR22691-219] - BSA and Azide free
See all CD38 primary antibodies -
Description
Rabbit monoclonal [EPR22691-219] to CD38 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF Mouse
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: Mouse splenocytes. Flow cyt: Mouse splenocytes.
-
General notes
Ab255693 is the carrier-free version of ab235590. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255693 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22691-219 -
Isotype
IgG -
Research areas
Images
-
Flow Cytometry - Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)
Flow cytometric analysis of mouse splenocytes labeling CD38 with ab235590 at 1/500 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab235590 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235590).
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)](https://www.abcam.com/ps/products/255/ab255693/Images/ab255693-1-anti-cd38-antibody-epr22691-219-immunocytochemistry-splenocytes-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD38 with ab235590 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of mouse splenocytes. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS nstead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235590).
-
![Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)](https://www.abcam.com/ps/products/255/ab255693/Images/ab255693-3-benefits-of-recombinant-antibodies.png)
Anti-CD38 antibody [EPR22691-219] - BSA and Azide free (ab255693)
