Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SFRP4 antibody [EPR9389] (ab154167)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9389] to SFRP4
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SFRP4 antibody [EPR9389]
    See all SFRP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR9389] to SFRP4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to residues within Human SFRP4 (UniProt: Q6FHJ7).

  • Positive control

    • Human ovary cancer, uterus cancer, MCF7 and 293T lysates; Human colon tissue, Human ovarian carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9389

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SFRP4 antibody [EPR9389] (ab154167)
    Western blot - Anti-SFRP4 antibody [EPR9389] (ab154167)
    All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution

    Lane 1 : Human ovary cancer lysate
    Lane 2 : Human uterus cancer lysate
    Lane 3 : MCF7 lysate
    Lane 4 : 293T lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (ab154167)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (ab154167)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SFRP4 with ab154167 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-SFRP4 antibody [EPR9389] (ab154167)
    Flow Cytometry - Anti-SFRP4 antibody [EPR9389] (ab154167)

    Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling SFRP4 with unpurified ab154167 at 1/130 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (ab154167)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (ab154167)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SFRP4 with ab154167 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-SFRP4 antibody [EPR9389] (ab154167)
    Anti-SFRP4 antibody [EPR9389] (ab154167)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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