Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)
![Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)](https://www.abcam.com/ps/products/240/ab240126/Images/ab240126-3-ab154167270673ab154167FC1.jpg "Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9389] to SFRP4 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SFRP4 antibody [EPR9389] - BSA and Azide free
See all SFRP4 primary antibodies -
Description
Rabbit monoclonal [EPR9389] to SFRP4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab240126 is the carrier-free version of ab154167. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240126 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9389 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)
Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling SFRP4 with unpurified ab154167 at 1/130 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154167).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)](https://www.abcam.com/ps/products/240/ab240126/Images/ab240126-2-SFRP4Primaryantibodiesab1541673.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SFRP4 with ab154167 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154167).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)](https://www.abcam.com/ps/products/240/ab240126/Images/ab240126-1-SFRP4Primaryantibodiesab1541674.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SFRP4 with ab154167 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154167).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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![Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)](https://www.abcam.com/ps/products/240/ab240126/Images/ab240126-4-benefits-of-recombinant-antibodies.png)
Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)
