Anti-IMPACT antibody (ab95175)
Key features and details
- Rabbit polyclonal to IMPACT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IMPACT antibody
See all IMPACT primary antibodies -
Description
Rabbit polyclonal to IMPACT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 270-320 (GILLGPDRFK HINNCARNIL VEKNYTNSPE ESSKALGKNK KVRKDKKRNE H) of Human IMPACT (NP_060909.1).
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Positive control
- HeLa and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab95175 was affinity purified using an epitope specific to IMPACT immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IMPACT antibody (ab95175)All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-IMPACT antibody (ab95175)Detection of IMPACT in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab95175 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml
Lane 1 : IP with IMPACT antibody
Lane 2 : IP with ab95175
Lane 3 : IP with control IgG
