Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)
![Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)](https://www.abcam.com/ps/products/75/ab75767/Images/ab75767-90827-anti-eif4ebp1-phospho-t37-antibody-epr7292y-western-blot.jpg "Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR729(2)Y] to eIF4EBP1 (phospho T37)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y]
See all eIF4EBP1 primary antibodies -
Description
Rabbit monoclonal [EPR729(2)Y] to eIF4EBP1 (phospho T37) -
Host species
Rabbit -
Specificity
Detects eIF4EBP1 only when phosphorylated at threonine 37. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
A phospho-peptide corresponding to residues surrounding threonine 37 of eIF4EBP1.
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Positive control
- 293 cells lysate.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR729(2)Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)All lanes : Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767) at 1/2000 dilution
Lane 1 : 293 cell lysate, untreated
Lane 2 : 293 cell lysate, treated with insulin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 13 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
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![Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)](https://www.abcam.com/ps/products/75/ab75767/Images/ab75767-2-benefits-of-recombinant-antibodies.png)
Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)
