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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)

Price and availability

251 280 ₸

Availability

Order now and get it on Tuesday March 23, 2021

Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR654(2)A] to eIF4EBP1 (phospho T70)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A]
    See all eIF4EBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR654(2)A] to eIF4EBP1 (phospho T70)
  • Host species

    Rabbit
  • Specificity

    ab75831 detects eIF4EBP1 phosphorylated at threonine 70.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 70 of human eIF4EBP1.

  • Positive control

    • 293T cell lysates treated with FBS.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR654(2)A
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

Images

  • Western blot - Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)
    Western blot - Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)
    All lanes : Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831) at 1/500 dilution

    Lane 1 : 293T cell lysates un-treated
    Lane 2 : 293T cell lysates treated with FBS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?

  • Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)
    Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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