Anti-eIF4EBP1 antibody (ab131453)
Key features and details
- Rabbit polyclonal to eIF4EBP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4EBP1 antibody
See all eIF4EBP1 primary antibodies -
Description
Rabbit polyclonal to eIF4EBP1 -
Host species
Rabbit -
Specificity
ab131453 detects endogenous levels of eIF4EBP1 protein. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide counjugated to KLH, surrounding amino acids 43-47 (S-T-T-P-G) of Human eIF4EBP1 (NP_004086.1).
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Positive control
- Methanol fixed HeLa cells; 293 and HepG2 cell extracts; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131453 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-eIF4EBP1 antibody (ab131453) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : HepG2 cell extracts
Predicted band size: 13 kDa
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Immunofluorescent analysis of methanol fixed HeLa cells labelling eIF4EBP1 with ab131453 antibody at a dilution of 1/100.
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling eIF4EBP1 with ab131453 at 1/50 dilution. The image on the right is treated with the synthesized peptide.