Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y330] to eIF4EBP1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-eIF4EBP1 antibody [Y330]
See all eIF4EBP1 primary antibodies
Description

Rabbit monoclonal [Y330] to eIF4EBP1
Host species

Rabbit
Specificity

ab32130 recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

A synthetic peptide corresponding to residues near the C-term of human4E-BP1
Positive control
- WB: HeLa, HEK-293 cell lysate, HAP1 and K562 cell lysate, IHC-P: Human prostate carcinoma
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

IgG fraction
Clonality

Monoclonal
Clone number

Y330
Isotype

IgG
Research areas

Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)

All lanes : Anti-eIF4EBP1 antibody [Y330] (ab32130) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EIF4EBP1 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 12 kDa
Observed band size: 13 kDa
why is the actual band size different from the predicted?

Lanes 1-2: Merged signal (red and green). Green - ab32130 observed at 13 kDa. Red - loading control ab8245 observed at 37 kDa.

ab32130 Anti-eIF4EBP1 antibody [Y330] was shown to specifically react with eIF4EBP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264784 (knockout cell lysate ab257146) was used. Wild-type and eIF4EBP1 knockout samples were subjected to SDS-PAGE. ab32130 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody [Y330] (ab32130)

ab32130, staining human prostate carcinoma by immunohistochemistry, Paraffin embedded tissue

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)

All lanes : Anti-eIF4EBP1 antibody [Y330] (ab32130) at 1/1000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : eIF4EBP1 knockout HAP1 cell lysate
Lane 3 : Hek293 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 12 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab32130 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32130 was shown to specifically react with eIF4EBP1 when eIF4EBP1 knockout samples were used. Wild-type and eIF4EBP1 knockout samples were subjected to SDS-PAGE. ab32130 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-eIF4EBP1 antibody [Y330] (ab32130)

Anti-eIF4EBP1 antibody [Y330] (ab32130) at 1/1000 dilution + K562 cell lysate

Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Anti-eIF4EBP1 antibody [Y330] (ab32130)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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