Anti-eIF4EBP1 (phospho T46) antibody (ab27792)
Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T46)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-eIF4EBP1 (phospho T46) antibody
See all eIF4EBP1 primary antibodies -
Description
Rabbit polyclonal to eIF4EBP1 (phospho T46) -
Host species
Rabbit -
Specificity
Ab27792 recognises eIF4EBP1 that is phosphorylated at threonine 46. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46. The sequence is conserved in human, mouse and rat.
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Positive control
- Human embryonic kidney (HEK293) cells stimulated with EGF or 15% serum, or NIH3T3 cells stimulated with PDGF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Ab27792 was purified from rabbit serum by sequential epitope specific chromatography. The antibody was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated eIF4EBP1. The final product is generated by affinity chromatography using a eIF4EBP1 derived peptide that is phosphorylated at threonine 46. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4EBP1 (phospho T46) antibody (ab27792)All lanes : Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two
hours at room temperature.)
Lane 1 : PDGF treated NIH3T3 cell lysate
Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate
Secondary
All lanes : goat F(ab)2 anti rabbit IgG
HRP conjugate
Predicted band size: 13 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
The figure shows that ab27792 can detect phosphorylated eIF4EBP1 in PDGF treated NIH3T3 cells (lane 1), but does not detect the unphosphorylated form (lanes 2 and 3: controls). Blocking step: 3% BSA TBST buffer for one hour at room temperature.
