Anti-eIF4EBP1 (phospho T36) antibody (ab47365)
Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T36)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4EBP1 (phospho T36) antibody
See all eIF4EBP1 primary antibodies -
Description
Rabbit polyclonal to eIF4EBP1 (phospho T36) -
Host species
Rabbit -
Specificity
ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).
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Positive control
- MDA-MB-435 cell extracts; Human breast carcinoma tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4EBP1 (phospho T36) antibody (ab47365)All lanes : Anti-eIF4EBP1 (phospho T36) antibody (ab47365)
Lane 1 : MDA-MB-435 cells,
treated with EGF (200 ng/ml, 30min)
Lane 2 : MDA-MB-435 cells, untreated
Predicted band size: 13 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 (phospho T36) antibody (ab47365)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47365. Left image without immunising peptide treatment; right image with immunising peptide treatment. -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T36) antibody (ab47365)ICC/IF image of ab47365 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
